DEVELOPMENT AND VALIDATION OF METHOD FOR THE ISOLATION AND QUANTITATIVE DETERMINATION OF FLAVONOIDS IN LEAVES OF SALVIA STEPPOSA DES.-SHOST.

UDC 543.42.062:582.6/.9:615.322

  • Kamilla Ildarovna Kashfullina Bashkir State Medical University https://orcid.org/0000-0002-7716-5706 Email: svet-khasanova@yandex.ru
  • Svetlana Rashitovna Khasanova Bashkir State Medical University Email: svet-khasanova@yandex.ru
  • Natalya Vladimirovna Kudashkina Bashkir State Medical University Email: phytoart@mail.ru
  • Timur Vilyrovich Bulgakov Moscow State University named after M.V. Lomonosov Email: svet-khasanova@yandex.ru
DOI_disc_logo https://doi.org/10.14258/jcprm.20250316389
Keywords: flavonoids, quantitative determination, Salvia stepposa Des.-Shost., extraction, validation, luteolin-7-glycoside

Abstract

The aim of our study is to develop optimal extraction conditions and validate a method for the quantitative determination of flavonoids in Salvia stepposa Des.–Shost. leaves. Upon completion of the study of the extraction parameters, optimal conditions for the extraction of flavonoids from S.stepposa leaves were determined: the grinding of raw materials – 1.0 – 2.0 mm, ethyl alcohol concentration – 60%, the ratio of raw materials and ethyl alcohol 1:200, AlCl3 concentration of 1%, followed by infusion for 30 minutes, a single extraction is sufficient for maximum extraction. The developed method has been tested for linearity, repeatability, reproducibility and correctness, and is a precision one.

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Author Biographies

Kamilla Ildarovna Kashfullina , Bashkir State Medical University

assistant of the pharmacology department

Svetlana Rashitovna Khasanova , Bashkir State Medical University

doctor of pharmaceutical sciences, professor, professor of the department of pharmacognosy and botany

Natalya Vladimirovna Kudashkina, Bashkir State Medical University

doctor of pharmaceutical sciences, professor, dean of the pharmaceutical faculty, head of the department of pharmacognosy and botany

Timur Vilyrovich Bulgakov, Moscow State University named after M.V. Lomonosov

candidate of pharmaceutical sciences, leading specialist of doping control of the National anti-doping laboratory

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Published
2025-09-15
How to Cite
1. Kashfullina K. I., Khasanova S. R., Kudashkina N. V., Bulgakov T. V. DEVELOPMENT AND VALIDATION OF METHOD FOR THE ISOLATION AND QUANTITATIVE DETERMINATION OF FLAVONOIDS IN LEAVES OF SALVIA STEPPOSA DES.-SHOST. // Chemistry of plant raw material, 2025. № 3. P. 167-175. URL: https://journal.asu.ru/cw/article/view/16389.
Issue
No 3 (2025)
Section
Low-molecular weight compounds

Copyright (c) 2025 chemistry of plant raw material

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