CHEMOTYPING AND STANDARDISATION OF ESSENTIAL OIL OF WINTER SAVORY (SATUREJA MONTANA L.) OF RUSSIAN ORIGIN
UDC 615.07, 615.322
Abstract
The objective of this study is to characterize the chemotype and standardize the essential oil of winter savory (Saturea Montana L.), a species of Russian origin. In order to standardize the essential oil, the current practices of essential oil standardization were studied and compared. Furthermore, the selection of standardization indicators and their normalization were carried out. The component composition was investigated using gas chromatography and gas chromatography-mass spectrometry. The essential oil of Russian winter savory was found to be characterized by the predominance of phenolic compounds in its composition, with carvacrol representing the main component. The content of carvacrol in the tested samples was found to be no less than 51.20%, which allowed us to classify Russian winter savory to the carvacrol chemotype. The quantitative determination of water by coulometric titration in accordance with the Karl Fischer method was tested for the purpose of standardizing essential oils. It was proposed that the determination of essential oil authenticity be carried out by means of a chromatographic profile in accordance with the ISO and European Pharmacopoeia approach.
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References
Gomes F., Dias M.I., Lima Â., Barros L., Rodrigues M.E., Ferreira I.C.F.R., Henriques M. Antibiotics, 2020, vol. 9, 294. https://doi.org/10.3390/antibiotics9060294.
Santos J.D.C. et al. Industrial Crops and Products, 2019, vol. 137, pp. 541–548. https://doi.org/10.1016/j.indcrop.2019.05.058.
Vitanza L. et al. Microbial Pathogenesis, 2019, vol. 126, pp. 323–331. https://doi.org/10.1016/j.micpath.2018.11.025.
Bezbradica D.I., Tomovic J.M., Vukasinovic M.S., Siler-Marinkovic S., Ristic M.M. Journal of Essential Oil Re-search, 2005, vol. 17(4), pp. 462–465. https://doi.org/10.1080/10412905.2005.9698965.
Svidenko L.V., Rabotyagov V.D., Khlypenko L.A. Byulleten' Gosudarstvennogo Nikitskogo botanicheskogo sada, 2006, no. 93, pp. 50–52. (in Russ.).
Miladi H. et al. Journal of Chemistry, 2013, vol. 1, 275698. https://doi.org/10.1155/2013/275698.
Radonic A., Milos M. Free Radical Research, 2003, vol. 37.6, pp. 673–679. https://doi.org/10.1080/1071576031000105643.
Sanja Ć., Šolić M.E., Maksimović M. Botanica serbica, 2013, vol. 37.2, pp. 159–165.
Marin M. et al. Flavour and Fragrance Journal, 2012, vol. 27.3, pp. 216–223. https://doi.org/10.1002/ffj.3082.
Navarro-Rocha J. et al. Industrial crops and products, 2020, vol. 145, 111958. https://doi.org/10.1016/j.indcrop.2019.111958.
Ibraliu A. et al. Journal of Medicinal Plants Research, 2010, vol. 4, pp. 1359–1364.
Slavkovska V., Jancic R., Milosavljevic S., Djokovic D. Journal of Essential Oil Research, 1997, vol. 9(6), pp. 629–634. https://doi.org/10.1080/10412905.1997.9700801.
Dodoš T. et al. Industrial Crops and Products, 2024, vol. 210, 118167. https://doi.org/10.1016/j.indcrop.2024.118167.
Gosudarstvennaya farmakopeya RF. XV izd. [State Pharmacopoeia of the Russian Federation. XV ed.]. Moscow, 2023. URL: https://pharmacopoeia.regmed.ru/pharmacopoeia/izdanie-15/. (in Russ.).
European Pharmacopoeia, 11th edition. European Department for the Quality and Medicines. Strasbourg, France, 2023.
ISO 11024-1:1998. Essential oils. General guidance on chromatographic profiles. Part 1: Preparation of chromato-graphic profiles for presentation in standards. 1998.
ISO 11024-2:1998. Essential oils. General guidance on chromatographic profiles. Part 2: Utilization of chromato-graphic profiles of samples of essential oils. 1998.
Adams R.P. Identification of essential oil components by gas chromatography/quadrupole mass spectroscopy ed. 4.1. Allured publishing corporation, 2017.
ISO 11021-1999. Essential oils. Determination of water content. Karl Fischer method. 1999.
Grünke S., Wünsch G. Fresenius J. Anal. Chem., 2000, vol. 368, pp. 139–147. https://doi.org/10.1007/s002160000444.
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