OBTAINING AND STUDYING PHYSICAL AND CHEMICAL PROPERTIES OF FILMS BASED ON COLLAGEN AND MELANIN
UDC 619:615.2:637
Abstract
The article is devoted to the production and study of the physicochemical properties of films based on collagen, Na-CMC and melanin. Solutions of compositions based on collagen, Na-CMC and melanin were obtained. By determining the dynamic viscosity of 1% solutions at 25 C, it was proven that at a collagen/Na-CMC mass ratio of 4/1 and 3/1, the native structure of collagen is preserved in the composition. Based on 1% polymer compositions, film samples containing 20, 50 and 70 mg of melanin were obtained. The resulting films are elastic light-brown plates with a smooth even surface 100–110 μm thick. It was found that with an increase in Na-CMC in the film composition, a sharp increase in the adhesive properties of the film samples was observed from 1729 to 2603 N × 10-3. At the same time, an increase in the amount of melanin in the film does not significantly affect its adhesive properties. To determine the level of bioresorbability of the film in the body, the time of their dissolution at a temperature of 37 °C was studied. It was found that the time of dissolution of the films increased with an increase in the content of Na-CMC and did not depend on the content of melanin. The effect of the sterilizing dose of radiation on the elasticity and time of dissolution in water of the films during sterilization was studied. The optimal composition of the film was established: collagen – 2 g, Na-CMC – 0.667 g, glycerin – 0.53 g, melanin - 50 mg, purified water to 100 g, which has the longest dissolution time – 230 min, ensuring maximum prolongation of the effect, optimal adhesive properties with an adhesion force of 2536 N × 10-3. When determining the acute toxicity of the film, when administered orally to mice in doses of 5000, 6000, 8000, 10000 mg/kg in the form of a 20% aqueous solution, no symptoms of acute poisoning were detected in any experimental animals, and there was no lethality (LD50 over 10000 mg/kg, class V of practically non-toxic compounds).
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