THE EFFICIENCY OF PAENIBACILLUS BACTERIA CULTIVATION ON NUTRIENT MEDIUM FROM ENZYMATIC HYDROLYSATE OF RICE HUSKS
UDC 602.44
Abstract
The full use of secondary raw materials from processing plants by biotechnological methods is economically feasible and promising for the biotechnological industry. The purpose of this study is developing technology for the utilization of rice husk to obtain nutrient medium for the cultivation of bacteria Paenibacillus, promising for the production of agricultural biological products, in particular, biological fertilizers and feed additives. The processing of rice husk by using chemical and biotechnological methods showed that optimized condition for the separation of fiber and mineral substances from rice husk is treating rice husk with sodium hydroxide with concentration 2.5% at 120 °C for 20 minutes. The possibility of obtaining simple sugars by enzymatic treatment of rice husk fiber with the enzyme preparation Accellerase 1500 for 24 hours at 55 °C was observed. Under these conditions, the enzymatic hydrolysate of rice husks contains 89% of reducing sugars of absolute dry matter, as well as amino acids and organic acids. It has been proved that the enzymatic hydrolysate of rice husk can be used as the main substrate for the cultivation of strains 560, 563, 567, 568, 572, 574, 17-2 bacteria P. mucilaginosus and 17-6 bacteria P. salinicaeni. Screening of bacteria Paenibacillus by specific growth rate, generation time, biomass yield, and also by the activity of extracellular enzymes, it was found that strain 560, which is recommended for further studies for developing technology of bioproducts for agricultural purposes.
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Morgunov I., Kamzolova S., Lunina J. Fermentation, 2018, vol. 4, no. 2, p. 36.
Cunha L., Martarello R., Souza P.M.D., Freitas M.M.D., Barros K.V.G., Ferreira Filho E.X., Magalhães P.O. Enzyme research, 2018, vol. 2018, 7 p.
Jadhav A.C., Pandit P., Gayatri T.N., Chavan P.P., Jadhav N.C. In Green Composites. Springer, Singapore, 2019, pp. 1–24.
Chandrasekhar S., Satyanarayana K.G., Pramada P.N., Raghavan P., Gupta T.N. Journal of Materials Science, 2003, vol. 38, no. 15, pp. 3159–3168.
Sarangi M., Bhattacharyya S., Behera R.C. Phase Transitions, 2009, vol. 82, no. 5, pp. 377–386.
Muntohar A.S. Civil Engineering Dimension, 2004, vol. 4, no. 2, pp. 100–105.
Houston D.F., Hill B.E., Garrett V.H., Kester E.B. Journal of Agricultural and Food Chemistry, 1963, vol. 11, no. 6, pp. 512–517.
Barber S., de Barber C.B. In Amino acid composition and biological value of cereal proteins. Springer, Dordrecht, 1985, pp. 481–494. DOI: 10.1007/978-94-009-5307-9_29.
Azat S., Korobeinyk A.V., Moustakas K., Inglezakis V.J. Journal of cleaner production, 2019, vol. 217, pp. 352–359.
Sapei L., Suseno N., Riadi L., Padmawijaya K.S., Thia S.G.W., Dewi V. Chemeca, 2018, p. 179.
Todkar B.S., Deorukhkar O.A., Deshmukh S.M. International Journal of Engineering Research and Development, 2016, vol. 12, no. 3, pp. 69–74.
Grady E.N., MacDonald J., Liu L., Richman A., Yuan Z.C. Microb. Cell. Fact., 2016, vol. 15, p. 203. DOI: 10.1186/s12934-016-0603-7.
Weselowski B., Nathoo N., Eastman A.W., MacDonald J., Yuan Z.C. BMC microbiology, 2016, vol. 16, no. 1, p. 244.
Wen Y., Wu X., Teng Y., Qian C., Zhan Z., Zhao Y. et al. Environ Microbiol., 2011, vol. 13, pp. 2726–2737.
Ha D.T., Kanarskiy A.V., Kanarskaya Z.A., Shcherbakov A.V., Shcherbakova Ye.N. Nauchnyy zhurnal NIU ITMO. Seriya «Protsessy i apparaty pishchevykh proizvodstv», 2019, vol. 3, pp. 62–72. (in Russ.).
Phi Q.T., Park Y.M., Seul K.J., Ryu C.M., Park S.H., Kim J.G. et al. Journal Microbiol Biotechnol., 2010, vol. 20, no. 12, pp. 1605–1613.
Sang M.K., Kim E.N., Han G.D., Kwack M.S., Jeun Y.C., Kim K.D. Phytopathology, 2014, vol. 104, no. 8, pp. 834–842.
Kumar S., Chauhan P.S., Agrawal L., Raj R., Srivastava A., Gupta S. et al. PLoS ONE, 2016, vol. 11, no. 3, e0149980.
Asha B.M., Revathi M., Yadav A., Sakthivel N. Journal of microbiology and biotechnology, 2012, vol. 22, no. 11, pp. 1501–1509.
Saxena N., Pore S., Arora P., Kapse N., Engineer A., Ranade D.R. et al. Biologia, 2015, vol. 70, no. 1, pp. 1–10.
Novia N., Pareek V.K., Hermansyah H., Jannah A.M. Science and Technology Indonesia, 2019, vol. 4, no. 1, pp. 18–23.
Nguyen M.H. Protsessy termicheskoy pererabotki risovoy shelukhi pri poluchenii aktivirovannogo uglerodnogo mate-riala i ikh apparaturnoye obespecheniye: avtoref. dis. …. kand. tekh. nauk. [The processes of thermal processing of rice husks upon receipt of activated carbon material and their hardware: abstract. dis. ... Cand. those. of sciences]. Tomsk, 2018, 24 p. (in Russ.).
da Silva Menezes B., Rossi D.M., Squina F., Ayub M.A.Z. Bioresource Technology Reports, 2018, vol. 2, pp. 100–106.
Madu J.O., Agboola B.O. 3 Biotech, 2018, vol. 8, no. 1, 15 p.
Morozova Yu.A., Skvortsov Ye.V., Alimova F.K., Kanarskiy A.V. Vestnik Kazanskogo tekhnologicheskogo universi-teta, 2012, vol. 15, no. 19, pp. 120–122. (in Russ.).
Yusupov R.A., Bakhteyev S.A., Gatiyatullin I.R. Vestnik Kazanskogo tekhnologicheskogo universiteta, 2011, vol. 19, pp. 306–308. (in Russ.).
Orata F. Advanced Gas Chromatography Progress in Agricultural, Biomedical and Industrial Applications, 2012, pp. 83–108.
Li X., Yang S.H., Yu X.C., Jin Z.X., Li W.D., Li L., Li M.G. Journal of applied microbiology, 2005, vol. 99, no. 4, pp. 878–884.
Koptyayev V.V., Sevast'yanova Yu.V., Dul'kin D.A., Kanarskiy A.V., Ha G.T., Kanarskaya Z.A., Yakubov Ye.R. Problemy mekhaniki tsellyulozno-bumazhnykh materialov: materialy mezhdunarodnoy nauchno-tekhnicheskoy konfer-entsii, posvyashchennoy pamyati professorov V.I. Komarova. [Problems of the mechanics of pulp and paper materials: materials of the international scientific and technical conference dedicated to the memory of professors V.I. Komarova]. Arkhangelsk, 2019, p. 341. (in Russ.).
Maier R.M. Bacterial Growth. In Environmental Microbiology. Elsevier Inc, 2009, pp. 37–54.
Adney B., Baker J. Measurement of cellulase activities. Laboratory analytical procedure. 1996, 11 p.
Bailey M., Biely J., Poutanen K. Journal Biotechnol., 1992, vol. 23, no. 3, pp. 257–270.
Dong M., Wang S., Xu F., Wang J., Yang N., Li, Q., Li W. Biotechnology for biofuels, 2019, vol. 12, no. 1, pр. 1–11.
Berg J.M., Tymoczko J.L., Stryer L. Biochemistry. 5th edition. New York: W H Freeman, 2002, 1050 p.
Stocks-Fischer S., Galinat J.K., Bang S.S. Soil Biology and Biochemistry, 1999, vol. 31, no. 11, pp. 1563–1571.
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