EXTRACTION OF FLAVONOIDS FROM EPIMEDIUM BREVICORNUM USING SUPERCRITICAL CARBON DIOX-IDE
Abstract
Advantages of supercritical extraction in comparison with other extract methods for active components from plant materials were discussed. The possibility of utilization of extraction with supercritical carbon dioxide for the isolation of biologically active substances from the leaves of Epimedium Brevicornum was demonstrated. The dependence of efficiency of isolation of flavonoids from experimental conditions (temperature, pressure, extraction, time) was investigated. A maximal extraction yield of active components was obtained under optimal conditions: a temperature of 50 °С, a pressure of 30 MPa and an extraction time of 30 min. The comparison of the contents of icariin, icariside II, epimedins A, B, C in the leaves of Epimedium Brevicornum extracts obtained by extraction with supercritical carbon dioxide and by ultrasound liquid extraction procedures was carried out by of liquid chromatography/mass spectrometry. The superiority of proposed method to traditional ultrasound liquid-liquid extraction in 2,5 times was demonstrated.
Downloads
Metrics
References
Флора CCCР. М., 1937. Т. 7. 718 с.
Энциклопедический словарь Ф.А. Брокгауза и И.А. Ефрона: Бесцветник. СПб., 1980. Т. 3А. №6. 956 с.
Editorial Committee of Pharmacopoeia of Ministry of Health PR China, The Pharmacopoeia of People’s Republic of China. Beijing, 2010. Vol. 1.
Huang X., Zhu D.Y., Lou Y.J. A novel anticancer agent, icaritin, induced cell growth inhibition, G1 arrest and mito-chondrial transmembrane potential drop in human prostate carcinoma PC-3 cells // Eur. J. Pharmacol. 2007. Vol. 564, N1-3. Pp. 26–36.
Humfrey C.D. Phytoestrogens and human health effects: weighing up the current evidence // Nat. Toxins. 1998. Vol. 6, N2. Pp. 51–59.
Felson D.T., Zhang Y., Hannan M.T., Kiel D.P., Wilson P.W., Anderson J.J. The effect of postmenopausal estrogen therapy on bone density in elderly women // N. Engl. J. Med. 1993. Vol. 329, N16. Pp. 1141–1146.
Pan Y., Kong L., Xia X., Zhang W., Xia Z., Jiang F. Antidepressant-like effect of icariin and its possible mechanism in mice // Pharmacol. Biochem. Behav. 2005. Vol. 82, N4. Pp. 686–694.
Wu J., Du J., Xu C., Le J., Xu Y., Liu B., Dong B. Icariin attenuates social defeat-induced down-regulation of gluco-corticoid receptor in mice // Pharmacol. Biochem. Behav. 2011. Vol. 98, N2. Pp. 273–278.
Ji H., Liu K., Li S.P., Gong X.J. Effect of HEF on osteoporosis induced by retinoic acid in male rats // J. Chin. Pharm. Univ. 2000. Vol. 31, N3. Pp. 222–225.
Meng F., Li Y.B., Xiong Z.L., Jiang Z.M., Li F.M. Osteoblastic proliferative activity of Epimedium brevicornum Max-im. // Phytomedicine. 2005. Vol. 12, N3. Pp. 189–193.
Ma A., Qi S., Xu D., Zhang X., Daloze P., Chen H. Baohuoside-1, a novel immunosuppressive molecule, inhibits lym-phocyte activation in vitro and in vivo // Transplantation. 2004. Vol. 78, N6. Pp. 831–838.
Cai M.L., Ji H., Li P., Wang M.Q. Effects of five flavonoids isolated from Epimedium pubescens on osteoblasts in vitro // Chin. J. Nat. Med. 2004. Vol. 2, N4. Pp. 235–238.
Wang Y., Guo Z., Jin Y., Zhang X., Wang L., Xue X., Liang X. Identification of prenyl flavonoid glycosides and phe-nolic acids in Epimedium koreanum Nakai by Q-TOF-MS combined with selective enrichment on «click oligo (ethylene glycol)» column // J. Pharm. and Biomed. Anal. 2010. Vol. 51, N3. Pp. 606–616.
Wu C.S., Guo B.L., Sheng Y.X., Zhang J.L. Simultaneous determination of seven flavonoids in Epimedium by liquid chromatography-tandem mass spectrometry method // Chinese Chemical Letters. 2008. Vol. 19. Pp. 329–332.
Chen X.J., Ji H., Zhang Q.W., Tu P.F., Wang Y.T., Guo B.L., Li S.P. A rapid method for simultaneous determination of 15 flavonoids in Epimedium using pressurized liquid extraction and ultra-performance liquid chromatography // J. Pharm. and Biomed. Anal. 2008. Vol. 46, N2. Pp. 226–235.
Xu Y., Li Z., Yuan L., Zhang X., Lu D., Huang H., Wang Y. Variation of Epimedins A C and Icariin in Ten Repre-sentative Populations of Epimedium brevicornu Maxim., and Implications for Utilization // Chemistry and Biodiversity. 2013. Vol. 10, N4. Pp. 711–721.
Li Y., Duan J., Guo T., Xie W., Yan S., Li B., Zhou Y., Chen Y. In vivo pharmacokinetics comparisons of icariin, emodin and psoralen from gan-kang granules and extracts of herba Epimedii, Nepal dock root, Ficus hirta yahl // J. Eth-nopharmacol. 2009. Vol. 124, N3. Pp. 522–529.
Могилюк В., Добровольный А. Сверхкритическая флюидная экстракция растительного сырья: перспективная технологическая платформа для фармацевтической промышленности // Фармацевтическая отрасль. 2015. T. 48, №1. С. 62–68.
Покровский О. Пробоподготовка в химическом анализе методом сверхкритической флюидной экстракции // Аналитика. 2013. T. 6, №13. С. 22–27.
Боголицын К.Г. Перспективы применения сверхкритических флюидных технологий в химии растительного сы-рья // Сверхкритические Флюиды: Теория и Практика. 2007. Т. 2, №1. С. 16–27.
Водяник А.Р., Шадрин А.Ю., Синев М.Ю. Сверхкритическая флюидная экстракция природного сырья: миро-вой опыт и ситуация в России // Сверхкритические Флюиды: Теория и Практика. 2008. Т. 3, №2. С. 58–69.
Zhang H.-F., Yang T.-S., Li Z.-Z., Wang Y. Simultaneous extraction of epimedin A, B, C and icariin from Herba Epimedii by ultrasonic technique // Ultrasonics Sonochemistry. 2008. Vol. 15, N4. Pp. 376–385.
This work is licensed under a Creative Commons Attribution 4.0 International License.
The authors, which are published in this journal, agree to the following conditions:
1. Authors retain the copyright to the work and transfer to the journal the right of the first publication along with the work, at the same time licensing it under the terms of the Creative Commons Attribution License, which allows others to distribute this work with the obligatory indication of the authorship of this work and a link to the original publication in this journal .
2. The authors retain the right to enter into separate, additional contractual agreements for the non-exclusive distribution of the version of the work published by this journal (for example, to place it in the university depository or to publish it in a book), with reference to the original publication in this journal.
3. Authors are allowed to post their work on the Internet (for example, in a university repository or on their personal website) before and during the review process of this journal, as this may lead to a productive discussion, as well as more links to this published work.
